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NASC

The European Arabidopsis Stock Centre

Wisconsin DsLox T-DNA lines

Donated by

  • Patrick Krysan Department of Horticulture, College of Agricultural and Life Sciences, University of Wisconsin, Madison
  • Richard Amasino Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin, Madison
  • Michael Sussman Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin, Madison
  • Joseph R. Ecker Plant Biology Laboratory, The Salk Institute for Biological Studies

Click here to view all 32664 of these lines.

Description

Wisconsin DsLox T-DNA lines

Generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector WiscDs-Lox. Basta herbicide was employed for selection of plants carrying a T-DNA. Each T1 transformant has been maintained individually. They can be utilised as in Ds Launchpad experiments. The T-DNA includes a Ds transposon/launchpad construction so that transposition can be induced by crossing to an Ac line. The selection of resulting transposants is by Basta and hygromycin. LoxP sites are also present so that regions between transposed Ds elements and the empty donor site can be subsequently removed by crossing to a Cre line.

The collection includes more than 30,000 lines.

WiscDsLox Seq Lines

New insertions have been identified for a number of the segregating SALK, SAIL and WiscDsLox lines. These insertions have been identified by TDNA-Seq, a next-generation sequencing method for capturing T-DNA flanking sequence tags developed in the Ecker laboratory.

Some of these have been isolated and donated as new lines available to order with NASC. These are noted on the parent WiscDsLox's stock page and are searchable through our quick search using the keyword ' Wiscseq* '.